Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3).

Article Details

Citation

Race JE, Grassl SM, Williams WJ, Holtzman EJ

Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3).

Biochem Biophys Res Commun. 1999 Feb 16;255(2):508-14.

PubMed ID
10049739 [ View in PubMed
]
Abstract

The cloned organic anion transporters from rat, mouse, and winter flounder (rOAT1, mOAT1, fROAT) mediate the coupled exchange of alpha-ketoglutarate with multiple organic anions, including p-aminohippurate (PAH). We have isolated two novel gene products from human kidney which bear significant homology to the known OATs and belong to the amphiphilic solute facilitator (ASF) family. The cDNAs, hOAT1 and hOAT3, encode for 550- and 568-amino-acid residue proteins, respectively. hOAT1 and hOAT3 mRNAs are expressed strongly in kidney and weakly in brain. Both genes map to chromosome 11 region q11.7. PAH uptake by Xenopus laevis oocytes injected with hOAT1 mRNA is increased 100-fold compared to water-injected oocytes. PAH uptake is chloride dependent and is not further increased by preincubation of oocytes in 5 mM glutarate. Uptake of PAH is inhibited by probenicid, alpha-ketoglutarate, bumetanide, furosemide, and losartan, but not by salicylate, urate, choline, amilioride, and hydrochlorothiazide.

DrugBank Data that Cites this Article

Drug Transporters
DrugTransporterKindOrganismPharmacological ActionActions
Aminohippuric acidSolute carrier family 22 member 8ProteinHumans
Unknown
Substrate
Inhibitor
Details
BumetanideSolute carrier family 22 member 6ProteinHumans
Unknown
Substrate
Inhibitor
Details
HydrochlorothiazideSolute carrier family 22 member 6ProteinHumans
Unknown
Substrate
Inhibitor
Details
LosartanSolute carrier family 22 member 6ProteinHumans
Unknown
Inhibitor
Details
Oxalic AcidSolute carrier family 22 member 8ProteinHumans
Unknown
Substrate
Details
ProbenecidSolute carrier family 22 member 6ProteinHumans
Unknown
Substrate
Inhibitor
Details
Salicylic acidSolute carrier family 22 member 6ProteinHumans
Unknown
Inhibitor
Details
Polypeptides
NameUniProt ID
Solute carrier family 22 member 6Q4U2R8Details
Solute carrier family 22 member 8Q8TCC7Details