Role of the sphingosine-1-phosphate receptor EDG-1 in PDGF-induced cell motility.

Article Details

Citation

Hobson JP, Rosenfeldt HM, Barak LS, Olivera A, Poulton S, Caron MG, Milstien S, Spiegel S

Role of the sphingosine-1-phosphate receptor EDG-1 in PDGF-induced cell motility.

Science. 2001 Mar 2;291(5509):1800-3.

PubMed ID
11230698 [ View in PubMed
]
Abstract

EDG-1 is a heterotrimeric guanine nucleotide binding protein-coupled receptor (GPCR) for sphingosine-1-phosphate (SPP). Cell migration toward platelet-derived growth factor (PDGF), which stimulates sphingosine kinase and increases intracellular SPP, was dependent on expression of EDG-1. Deletion of edg-1 or inhibition of sphingosine kinase suppressed chemotaxis toward PDGF and also activation of the small guanosine triphosphatase Rac, which is essential for protrusion of lamellipodia and forward movement. Moreover, PDGF activated EDG-1, as measured by translocation of beta-arrestin and phosphorylation of EDG-1. Our results reveal a role for receptor cross-communication in which activation of a GPCR by a receptor tyrosine kinase is critical for cell motility.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Sphingosine 1-phosphate receptor 1P21453Details