Molecular characterization of human and rat organic anion transporter OATP-D.

Article Details

Citation

Adachi H, Suzuki T, Abe M, Asano N, Mizutamari H, Tanemoto M, Nishio T, Onogawa T, Toyohara T, Kasai S, Satoh F, Suzuki M, Tokui T, Unno M, Shimosegawa T, Matsuno S, Ito S, Abe T

Molecular characterization of human and rat organic anion transporter OATP-D.

Am J Physiol Renal Physiol. 2003 Dec;285(6):F1188-97.

PubMed ID
14631946 [ View in PubMed
]
Abstract

We have isolated and characterized a novel human and rat organic anion transporter subtype, OATP-D. The isolated cDNA from human brain encodes a polypeptide of 710 amino acids (Mr 76,534) with 12 predicted transmembrane domains. The rat clone encodes 710 amino acids (Mr 76,821) with 97.6% amino acid sequence homology with human OATP-D. Human and rat OATP-D have moderate amino acid sequence homology with LST-l/rlst-1, the rat oatp family, the prostaglandin transporter, and moatl/MOAT1/KIAA0880/OATP-B. Phylogenetic tree analysis revealed that OATP-D is branched in a different position from all known organic anion transporters. OATP-D transports prostaglandin E1 (Km 48.5 nM), prostaglandin E2 (Km 55.5 nM), and prostaglandin F2,, suggesting that, functionally, OATP-D encodes a protein that has similar characteristics to those of the prostaglandin transporter. Rat OATP-D also transports prostaglandins. The expression pattern of OATP-D mRNA was abundant mainly in the heart, testis, brain, and some cancer cells. Immunohistochemical analysis further revealed that rat OATP-D is widely expressed in the vascular, renal, and reproductive system at the protein level. These results suggest that OATP-D plays an important role in translocating prostaglandins in specialized tissues and cells.

DrugBank Data that Cites this Article

Drug Transporters
DrugTransporterKindOrganismPharmacological ActionActions
AlprostadilSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Inhibitor
Details
Aminohippuric acidSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Inhibitor
Details
Dinoprost tromethamineSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Not AvailableDetails
DinoprostoneSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Substrate
Details
IloprostSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Substrate
Details
MethotrexateSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Substrate
Details
Taurocholic acidSolute carrier organic anion transporter family member 3A1ProteinHumans
Unknown
Inhibitor
Details