Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).
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Lu R, Kanai N, Bao Y, Schuster VL
Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).
J Clin Invest. 1996 Sep 1;98(5):1142-9.
- PubMed ID
- 8787677 [ View in PubMed]
- Abstract
We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.
DrugBank Data that Cites this Article
- Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Alprostadil Solute carrier organic anion transporter family member 2A1 Protein Humans UnknownSubstrateDetails Dinoprost tromethamine Solute carrier organic anion transporter family member 2A1 Protein Humans UnknownNot Available Details Dinoprostone Solute carrier organic anion transporter family member 2A1 Protein Humans UnknownSubstrateDetails Iloprost Solute carrier organic anion transporter family member 2A1 Protein Humans UnknownSubstrateDetails Prostaglandin D2 Solute carrier organic anion transporter family member 2A1 Protein Humans UnknownNot Available Details - Polypeptides
Name UniProt ID Solute carrier organic anion transporter family member 2A1 Q92959 Details