Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).

Article Details

Citation

Lu R, Kanai N, Bao Y, Schuster VL

Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).

J Clin Invest. 1996 Sep 1;98(5):1142-9.

PubMed ID
8787677 [ View in PubMed
]
Abstract

We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.

DrugBank Data that Cites this Article

Drug Transporters
DrugTransporterKindOrganismPharmacological ActionActions
AlprostadilSolute carrier organic anion transporter family member 2A1ProteinHumans
Unknown
Substrate
Details
Dinoprost tromethamineSolute carrier organic anion transporter family member 2A1ProteinHumans
Unknown
Not AvailableDetails
DinoprostoneSolute carrier organic anion transporter family member 2A1ProteinHumans
Unknown
Substrate
Details
IloprostSolute carrier organic anion transporter family member 2A1ProteinHumans
Unknown
Substrate
Details
Prostaglandin D2Solute carrier organic anion transporter family member 2A1ProteinHumans
Unknown
Not AvailableDetails
Polypeptides
NameUniProt ID
Solute carrier organic anion transporter family member 2A1Q92959Details