Identification of the cytoskeletal protein alpha-actinin as a platelet thrombospondin-binding protein.

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Citation

Dubernard V, Faucher D, Launay JM, Legrand C

Identification of the cytoskeletal protein alpha-actinin as a platelet thrombospondin-binding protein.

FEBS Lett. 1995 May 8;364(2):109-14.

PubMed ID
7750553 [ View in PubMed
]
Abstract

Binding of the alpha-granular thrombospondin (TSP) to the plasma membrane of activated platelets has long been documented, yet the molecular mechanism involved in its secretion and surface expression have not been elucidated. Using a ligand blot binding assay where electrophoretically separated platelet proteins were incubated with purified 125I-labeled TSP, we observed a strong interaction of [125I]TSP with a 100 kDa single chain protein. On performing a platelet subfractionation, the 100 kDa protein was predominantly localized in the cytosol from which it was purified by preparative electrophoresis and was identified by amino acid sequencing to the cytoskeletal protein, alpha-actinin. We further demonstrated that [125I]TSP interacts with alpha-actinin in a specific manner and with a high affinity (Kd = 6.6 nM) in a solid-phase binding assay.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Alpha-actinin-1P12814Details