Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport.
Article Details
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Sulzer D, Chen TK, Lau YY, Kristensen H, Rayport S, Ewing A
Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport.
J Neurosci. 1995 May;15(5 Pt 2):4102-8.
- PubMed ID
- 7751968 [ View in PubMed]
- Abstract
Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole-cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from PC12 cells by > 50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport, and dopamine release.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Amphetamine Synaptic vesicular amine transporter Protein Humans YesInhibitorDetails Benzphetamine Synaptic vesicular amine transporter Protein Humans YesInducerDetails Dextroamphetamine Synaptic vesicular amine transporter Protein Humans YesInducerDetails Isometheptene Synaptic vesicular amine transporter Protein Humans YesInhibitorDetails Metamfetamine Synaptic vesicular amine transporter Protein Humans YesInhibitorDetails - Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Ephedrine Synaptic vesicular amine transporter Protein Humans UnknownInhibitorDetails