Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616.

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Licht J, Gally D, Henderson T, Young K, Cooper S

Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616.

Res Microbiol. 1993 Jul-Aug;144(6):423-33.

PubMed ID

8190989 [ View in PubMed

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Abstract

The effects of mecillinam, ampicillin and cephalexin on peptidoglycan synthesis in Salmonella typhimurium 2616 have been studied at equivalent concentrations or "isoactivities". Using antibiotics at isoactivities allows a direct comparison of the biochemical effects of different antibiotics. When mecillinam was added at different times during the division cycle at a concentration that produced 50% inhibition of peptidoglycan synthesis in an exponential culture over a short period of time, the inhibition of synthesis was greatest in the newborn cells and least in the dividing cells. Antibiotic competition experiments showed that mecillinam preferentially bound to penicillin-binding protein 2 in S. typhimurium 2616. High performance liquid chromatography analysis of the residual peptidoglycan synthesized in the presence of mecillinam showed an unexpected increase in pentapeptides and a significant increase in cross-linking. Other antibiotics added at equivalent activities did not show an increase in cross-linking.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Amdinocillin	Penicillin-binding protein 2	Protein	Escherichia coli (strain K12)	Yes	Inhibitor	Details