Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
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Hock J, Weinmann L, Ender C, Rudel S, Kremmer E, Raabe M, Urlaub H, Meister G
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
EMBO Rep. 2007 Nov;8(11):1052-60. Epub 2007 Oct 12.
- PubMed ID
- 17932509 [ View in PubMed]
- Abstract
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.