Preferential deimination of keratin K1 and filaggrin during the terminal differentiation of human epidermis.

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Citation

Senshu T, Kan S, Ogawa H, Manabe M, Asaga H

Preferential deimination of keratin K1 and filaggrin during the terminal differentiation of human epidermis.

Biochem Biophys Res Commun. 1996 Aug 23;225(3):712-9.

PubMed ID
8780679 [ View in PubMed
]
Abstract

The upper layers of mammalian epidermis contain citrulline-containing proteins formed by enzymatic deimination of arginine residues. To study the role of protein deimination in epidermal differentiation, we identified deiminated proteins extracted from human epidermis. Major deiminated proteins were identified as partially degraded keratin K1, while those from keratin K10 and a highly heterogeneous mixture of deiminated filaggrin isomers were detected as minor components. Deiminated keratins were recovered in a fraction enriched with keratins from the cornified layers. The subsequent immunohistochemical study showed that deiminated proteins were localized mainly in the lowermost cornified layer, but not in the granular layer. These data suggested that partially degraded/disulfide-cross-linked keratin K1 was preferentially deiminated during the terminal stages of epidermal differentiation. We therefore speculated that the protein deimination might influence the interaction of basic K1 with its acidic partner K10, pre-existent K5/K14 networks or keratin-associated protein filaggrin.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Keratin, type II cytoskeletal 1P04264Details