Detection of the damage caused to DNA by niclosamide using an electrochemical DNA-biosensor.

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Abreu FC, Goulart MO, Brett AM

Detection of the damage caused to DNA by niclosamide using an electrochemical DNA-biosensor.

Biosens Bioelectron. 2002 Dec;17(11-12):913-9.

PubMed ID

12392939 [ View in PubMed

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Abstract

Niclosamide is the only commercially available molluscicide recommended by the WHO for large-scale use in schistosomiasis control programs. The electrochemical reduction and oxidation mechanism of niclosamide was studied using cyclic, differential and square wave voltammetry, at a glassy carbon electrode. An indirect procedure for in situ quantification of niclosamide using batch injection analysis with electrochemical detection, possible to be used for in situ determinations in river streams and effluents, was developed. It enabled a detection limit of 8 x 10(-7) M. The investigation of the niclosamide-DNA interaction using an electrochemical DNA-biosensor showed for the first time clear evidence of interaction with DNA and suggested that niclosamide toxicity can be caused by this interaction, after reductive activation.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Niclosamide	DNA	Nucleotide	Humans	Unknown	Antagonist	Details