Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B12.

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Fang H, Li D, Kang J, Jiang P, Sun J, Zhang D

Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B12.

Nat Commun. 2018 Nov 21;9(1):4917. doi: 10.1038/s41467-018-07412-6.

PubMed ID

30464241 [ View in PubMed

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Abstract

The only known source of vitamin B12 (adenosylcobalamin) is from bacteria and archaea. Here, using genetic and metabolic engineering, we generate an Escherichia coli strain that produces vitamin B12 via an engineered de novo aerobic biosynthetic pathway. In vitro and/or in vivo analysis of genes involved in adenosylcobinamide phosphate biosynthesis from Rhodobacter capsulatus suggest that the biosynthetic steps from co(II)byrinic acid a,c-diamide to adocobalamin are the same in both the aerobic and anaerobic pathways. Finally, we increase the vitamin B12 yield of a recombinant E. coli strain by more than approximately 250-fold to 307.00 microg g(-1) DCW via metabolic engineering and optimization of fermentation conditions. Beyond our demonstration of E. coli as a microbial biosynthetic platform for vitamin B12 production, our study offers an encouraging example of how the several dozen proteins of a complex biosynthetic pathway can be transferred between organisms to facilitate industrial production.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Cyanocobalamin	Bifunctional adenosylcobalamin biosynthesis protein CobU	Protein	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	Unknown	Product of	Details