Improved high-performance liquid chromatographic determination of ciprofloxacin and its metabolites in human specimens.

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Mack G

Improved high-performance liquid chromatographic determination of ciprofloxacin and its metabolites in human specimens.

J Chromatogr. 1992 Nov 6;582(1-2):263-7.

PubMed ID
1491053 [ View in PubMed
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Abstract

A simple approach to the quantitation of ciprofloxacin and its three metabolites, M1 (desethylene-ciprofloxacin), M2 (sulfo-ciprofloxacin) and M3 (oxo-ciprofloxacin), in human serum, urine, saliva and sputum is described. This assay allows the parent drug and its metabolites to elute and be resolved in a single chromatogram at 280 nm using a linear gradient. The procedure involved liquid-liquid extraction. Separation was achieved on a C18 reversed-phase column. The limit of detection of ciprofloxacin is 0.05 microgram/ml and that of its three metabolites is 0.25 microgram/ml. This method is sufficiently sensitive for pharmacokinetic studies.

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