Metabolism of trimipramine in vitro by human CYP2D6 isozyme.

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Bolaji OO, Coutts RT, Baker GB

Metabolism of trimipramine in vitro by human CYP2D6 isozyme.

Res Commun Chem Pathol Pharmacol. 1993 Oct;82(1):111-20.

PubMed ID

8272570 [ View in PubMed

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Abstract

In vitro metabolism of the tricyclic antidepressant trimipramine using a commercial preparation of human CYP2D6 isozyme expressed in a human cell line is described. 2-Hydroxytrimipramine and a previously unreported metabolite, 2,10- or 2,11-dihydroxytrimipramine were isolated. Their structures were determined by gas chromatography/mass spectroscopy of underivatized and derivatized extracts. Acetylation of the new metabolite resulted in dehydration at C10 to give 10,11-dehydro-2-acetoxytrimipramine. No N-dealkylation of trimipramine was observed. Prior administration of quinidine produced a large reduction in the metabolic oxidation of trimipramine with CYP2D6 while prior administration of quinine had no effect. The use of this CYP2D6 isozyme preparation in vitro is of value in the identification of possible in vivo substrates for the human CYP2D6 isozyme.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Trimipramine	Cytochrome P450 2D6	Protein	Humans	Unknown	Substrate	Details