Specific cleavages of DNA by ascorbate in the presence of copper ion or copper chelates.

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Citation

Chiou SH, Chang WC, Jou YS, Chung HM, Lo TB

Specific cleavages of DNA by ascorbate in the presence of copper ion or copper chelates.

J Biochem. 1985 Dec;98(6):1723-6. doi: 10.1093/oxfordjournals.jbchem.a135445.

PubMed ID
3937842 [ View in PubMed
]
Abstract

The DNA-cleavage specificity of ascorbate in the presence of copper ion is analyzed with end-labeled pBR322 DNA fragments. The nonenzymatic reaction of Cu(II)/ascorbate and DNA shows certain degrees of cleavage preference toward purine-containing short segments in the labeled DNA under mild conditions (at 0 degrees C and 10 min). The segments of pyrimidine clusters are least susceptible to cleavage. The DNA scission cannot be detected in the absence of metal ions, and is greatly diminished in the presence of EDTA and metal-chelating peptide. It is more specific than the nuclease-like scission activity induced by cuprous-phenanthroline complex. This scission activity in relation to the antiviral and antitumor activities of vitamin C reported in the literature deserves a crucial consideration.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
Ascorbic acidDNANucleotideHumans
Unknown
Cleavage
Details