Expression and functional characterization of sugar beet phosphoethanolamine/phosphocholine phosphatase under salt stress.

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Sahashi K, Yamada-Kato N, Maeda T, Kito K, Cha-Um S, Rai V, Tanaka Y, Takabe T

Expression and functional characterization of sugar beet phosphoethanolamine/phosphocholine phosphatase under salt stress.

Plant Physiol Biochem. 2019 Sep;142:211-216. doi: 10.1016/j.plaphy.2019.07.011. Epub 2019 Jul 9.

PubMed ID

31302410 [ View in PubMed

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Abstract

Choline is a vital metabolite in plant and synthesized from phosphocholine by phosphocholine phosphatase. The Arabidopsis At1g17710 was identified as the first plant gene encoding the phosphatase for both phosphoethanolamine and phosphocholine (PECP) with much higher catalytic efficiency (>10-fold) for former. In betaine accumulating plants, choline is further required for betaine synthesis. In this report, we found three putative PECP genes in sugar beet, betaine accumulating plants. Two genes encode the proteins of 274 amino acid residues and designated as BvPECP1S and BvPECP2S. Another gene encodes the 331 amino acid protein (BvPECP2L) consisted of BvPECP2S with extra C-terminal amino acid. Enzymatic assays of BvPECP1S revealed that BvPECP1S exhibited the phosphatase activity for both phosphoethanolamine and phosphocholine with higher affinity (>1.8-fold) and catalytic efficiency (>2.64-fold) for phosphocholine. BvPECP2L exhibited low activity. RT-PCR experiments for BvPECP1S showed the increased expression in young leaf and root tip under salt-stress whereas the increased expression in all organs under phosphate deficiency. The expression level of BvPECP2L in salt stressed young leaf and root tip was induced by phosphate deficient. Physiological roles of BvPECP1S and BvPECP2L for the betaine synthesis were discussed.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Choline	Phosphoethanolamine/phosphocholine phosphatase	Protein	Humans	Unknown	Product of	Details