The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.

Article Details

Citation

Nestler M, Martin U, Hortschansky P, Saluz HP, Henke A, Munder T

The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.

Mol Cell Biochem. 2006 Jul;287(1-2):147-55. Epub 2006 May 9.

PubMed ID
16683188 [ View in PubMed
]
Abstract

Host answers to pathogen attacks define the course of pathogenic events and decide about the fate of the host organism. Infection with coxsackievirus B3 (CVB3) can induce severe myocarditis and pancreatitis. The interplay between host factors and virus components is crucial for the fate of the infected host. As we have shown before, expression of the pro-apoptotic host protein Siva is significantly increased after CVB3 infection, and infected cells are removed by programmed cell death. Analysis of Siva expressed in Escherichia coli revealed that this protein binds three zinc ions, suggesting a rather complex three-dimensional structure. By screening a human heart cDNA library we found a new interaction partner of Siva. The peroxisomal membrane protein PMP22 may be involved in the host response against CVB3. Previous investigations showed that Siva interacts with the cytoplasmic C-terminus of CD27, a member of the tumor necrosis factor receptor group, and transmits an apoptotic signal. With the help of directed two-hybrid assays we determined the N-terminal part of Siva as the binding region for CD27.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
ZincApoptosis regulatory protein SivaProteinHumans
Unknown
Not AvailableDetails
Zinc acetateApoptosis regulatory protein SivaProteinHumans
Unknown
Not AvailableDetails
Zinc chlorideApoptosis regulatory protein SivaProteinHumans
Unknown
Binder
Details
Zinc sulfate, unspecified formApoptosis regulatory protein SivaProteinHumans
Unknown
Binder
Details
Polypeptides
NameUniProt ID
Apoptosis regulatory protein SivaO15304Details