P300 transcriptional repression is mediated by SUMO modification.

Article Details

Citation

Girdwood D, Bumpass D, Vaughan OA, Thain A, Anderson LA, Snowden AW, Garcia-Wilson E, Perkins ND, Hay RT

P300 transcriptional repression is mediated by SUMO modification.

Mol Cell. 2003 Apr;11(4):1043-54. doi: 10.1016/s1097-2765(03)00141-2.

PubMed ID
12718889 [ View in PubMed
]
Abstract

p300 and CREB binding protein can both activate and repress transcription. Here, we locate the CRD1 transcriptional repression domain between residues 1017 and 1029 of p300. This region contains two copies of the sequence psiKxE that are modified by the ubiquitin-like protein SUMO-1. Mutations that reduce SUMO modification increase p300-mediated transcriptional activity and expression of a SUMO-specific protease or catalytically inactive Ubc9 relieved repression, demonstrating that p300 repression was mediated by SUMO conjugation. SUMO-modified CRD1 domain bound HDAC6 in vitro, and p300 repression was relieved by histone deacetylase inhibition and siRNA-mediated ablation of HDAC6 expression. These results reveal a mechanism controlling p300 function and suggest that SUMO-dependent repression is mediated by recruitment of HDAC6.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Histone acetyltransferase p300Q09472Details