A ratiometric fluorescent biosensor for the sensitive determination of alpha-glucosidase activity and acarbose based on N-doped carbon dots.
Article Details
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Wang M, Wang M, Zhang F, Su X
A ratiometric fluorescent biosensor for the sensitive determination of alpha-glucosidase activity and acarbose based on N-doped carbon dots.
Analyst. 2020 Aug 24;145(17):5808-5815. doi: 10.1039/d0an01065k.
- PubMed ID
- 32672281 [ View in PubMed]
- Abstract
In this work, a novel ratiometric fluorescent platform for alpha-glucosidase (alpha-glu) and its inhibitor was constructed based on N-doped carbon dots (N-CDs). The alpha-glucosidase present can catalyze the release of hydroquinone (HQ) from alpha-arbutin. Then, the generated HQ can be oxidized and copolymerized with polyethyleneimine (PEI) to form a yellowish green fluorescence copolymer (PHQ-PEI) with intense fluorescence emission at 510 nm. When the PHQ-PEI was formed, blue fluorescence of N-CDs at 425 nm was decreased, whereas the fluorescence of PHQ-PEI at 510 nm increased sharply as a result of the fluorescence resonance energy transfer (FRET) effect between N-CDs and PHQ-PEI. However, in the presence of acarbose, the activity of alpha-glucosidase is inhibited, and alpha-arbutin cannot be hydrolyzed to hydroquinone, leading to the fluorescence recovery of N-CDs at 425 nm and the fluorescence decrease of PHQ-PEI at 510 nm. The linear range from 0.2 to 1.6 mU mL-1 and 25-150 mumol L-1 was obtained for alpha-glucosidase and acarbose detection, respectively, and the detection limit (LOD) for alpha-glucosidase and acarbose was as low as 0.082 mU mL-1 and 14.5 mumol L-1. Thus, a ratiometric fluorescent sensor with good sensitivity and high specificity was established for alpha-glucosidase assay and satisfactory results were acquired in real sample determination.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Acarbose Lysosomal alpha-glucosidase Protein Humans UnknownInhibitorDetails alpha-Arbutin Lysosomal alpha-glucosidase Protein Humans UnknownSubstrateRegulatorDetails - Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Hydroquinone Lysosomal alpha-glucosidase Protein Humans UnknownProduct ofDetails