Reversal by aminoguanidine of the inhibition of proliferation of human fibroblasts by spermidine and spermine.

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Gahl WA, Pitot HC

Reversal by aminoguanidine of the inhibition of proliferation of human fibroblasts by spermidine and spermine.

Chem Biol Interact. 1978 Jul;22(1):91-8. doi: 10.1016/0009-2797(78)90152-7.

PubMed ID

99252 [ View in PubMed

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Abstract

The inhibitory effect of the polyamines, spermidine and spermine, on the proliferation of human fibroblasts in culture was found to be reversed by the addition of aminoguanidine (AM), a specific and highly effective inhibitor of diamine oxidase (DAO) present in fetal calf serum (FCS). Aminoguanidine itself in concentration as high as 10(-3) M exhibited no effect upon cell proliferation nor did putrescine at similar concentrations. However, at higher concentrations of putrescine, cell proliferation was inhibited and this inhibition was unaffected by the addition of mM concentrations of AM. These studies support earlier hypotheses on the mechanisms of the toxic effects of polyamines on cell proliferation and establish further that the diamine oxidase-catalyzed metabolism of spermine and spermidine is necessary for their toxic effects in cell culture.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Pimagedine	Amiloride-sensitive amine oxidase [copper-containing]	Protein	Humans	Yes	Inhibitor	Details