Effect of prolonged in vivo administration of progesterone in pregnancy on myometrial gene expression, peripheral blood leukocyte activation, and circulating steroid hormone levels.
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Norman JE, Yuan M, Anderson L, Howie F, Harold G, Young A, Jordan F, McInnes I, Harnett MM
Effect of prolonged in vivo administration of progesterone in pregnancy on myometrial gene expression, peripheral blood leukocyte activation, and circulating steroid hormone levels.
Reprod Sci. 2011 May;18(5):435-46. doi: 10.1177/1933719110395404.
- PubMed ID
- 21558462 [ View in PubMed]
- Abstract
OBJECTIVE: We aimed to investigate the effects of progesterone on gene expression and function of both myometrium and circulating leukocytes. METHODS: We recruited women participating in a randomized clinical trial of progesterone to prevent preterm delivery. These participants had a twin pregnancy and were managed in 1 of 2 tertiary referral centers. Participants were treated with progesterone (90 mg vaginally) or placebo from 24 to 34 weeks of pregnancy. The outcome measures were myometrial and leukocyte gene expression and expression of cell surface markers in circulating leukocytes, all quantified ex vivo. RESULTS: Prolonged in vivo administration of progesterone inhibited myometrial expression of connexins 26 and 43, endothelial nitric acid synthase (eNOS), and the prostaglandin receptor EP2 ex vivo. Administration of progesterone also increased numbers of circulating neutrophils while decreasing lymphocyte proportions and decreasing neutrophil CD11b expression. CONCLUSION: The observed effects of prolonged in vivo administration of progesterone will minimize the ability of the uterus to contract as a synctium and the ability of peripheral blood leukocytes to migrate into the myometrium during parturition. We suggest that these are putative mechanisms by which progesterone might prevent preterm birth in women at high risk.
DrugBank Data that Cites this Article
- Pharmaco-proteomics
Drug Drug Groups Gene Gene ID Change Interaction Chromosome Progesterone Approved Vet Approved GJA1 2697 decreased Progesterone results in decreased expression of GJA1 protein 6q22.31 Progesterone Approved Vet Approved GJB2 2706 decreased Progesterone results in decreased expression of GJB2 protein 13q12.11 Progesterone Approved Vet Approved ITGAM 3684 decreased Progesterone results in decreased expression of ITGAM protein 16p11.2 Progesterone Approved Vet Approved NOS3 4846 decreased Progesterone results in decreased expression of NOS3 protein 7q36.1 Progesterone Approved Vet Approved PTGER2 5732 decreased Progesterone results in decreased expression of PTGER2 protein 14q22.1