Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning.

Article Details

Citation

He QL, Jiang H, Zhang F, Chen HB, Tang GL

Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning.

Bioorg Med Chem Lett. 2008 Jul 15;18(14):3995-8. doi: 10.1016/j.bmcl.2008.06.006. Epub 2008 Jun 6.

PubMed ID
18571405 [ View in PubMed
]
Abstract

Simultaneously isolating more than one receptor of natural product remains a challenge to chemical genetics. Using cyclosporine A as an affinity probe and an optimized phage display cloning procedure, not only cyclophilin A, but also cyclophilin B was isolated as the full-length gene clone from a human brain cDNA library. This optimized protocol can be used to select protein targets of chemicals dependent on the binding affinity rather than on the relative abundance in cells.

DrugBank Data that Cites this Article

Binding Properties
DrugTargetPropertyMeasurementpHTemperature (°C)
CyclosporinePeptidyl-prolyl cis-trans isomerase AKd (nM)36.8N/AN/ADetails