Virtual and biomolecular screening converge on a selective agonist for GPR30.
Article Details
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Bologa CG, Revankar CM, Young SM, Edwards BS, Arterburn JB, Kiselyov AS, Parker MA, Tkachenko SE, Savchuck NP, Sklar LA, Oprea TI, Prossnitz ER
Virtual and biomolecular screening converge on a selective agonist for GPR30.
Nat Chem Biol. 2006 Apr;2(4):207-12. Epub 2006 Mar 5.
- PubMed ID
- 16520733 [ View in PubMed]
- Abstract
Estrogen is a hormone critical in the development, normal physiology and pathophysiology of numerous human tissues. The effects of estrogen have traditionally been solely ascribed to estrogen receptor alpha (ERalpha) and more recently ERbeta, members of the soluble, nuclear ligand-activated family of transcription factors. We have recently shown that the seven-transmembrane G protein-coupled receptor GPR30 binds estrogen with high affinity and resides in the endoplasmic reticulum, where it activates multiple intracellular signaling pathways. To differentiate between the functions of ERalpha or ERbeta and GPR30, we used a combination of virtual and biomolecular screening to isolate compounds that selectively bind to GPR30. Here we describe the identification of the first GPR30-specific agonist, G-1 (1), capable of activating GPR30 in a complex environment of classical and new estrogen receptors. The development of compounds specific to estrogen receptor family members provides the opportunity to increase our understanding of these receptors and their contribution to estrogen biology.
DrugBank Data that Cites this Article
- Binding Properties
Drug Target Property Measurement pH Temperature (°C) Estradiol Estrogen receptor alpha Ki (nM) 0.3 N/A N/A Details Estradiol Estrogen receptor beta Ki (nM) 0.38 N/A N/A Details Estradiol G-protein coupled estrogen receptor 1 Ki (nM) 5.7 N/A N/A Details Estradiol G-protein coupled estrogen receptor 1 EC 50 (nM) 0.3 N/A N/A Details