Estrogenic activity of chemical constituents from Tephrosia candida.

Article Details

Citation

Hegazy ME, El-Hamd H Mohamed A, El-Halawany AM, Djemgou PC, Shahat AA, Pare PW

Estrogenic activity of chemical constituents from Tephrosia candida.

J Nat Prod. 2011 May 27;74(5):937-42. doi: 10.1021/np100378d. Epub 2011 Apr 21.

PubMed ID
21510635 [ View in PubMed
]
Abstract

In a continued investigation of medicinal plants from the genus Tephrosia, phytochemical analysis of a methylene chloride-methanol (1:1) extract of the air-dried aerial parts of Tephrosia candida afforded two new 8-prenylated flavonoids, namely, tephrocandidins A (1) and B (2), a new prenylated chalcone, candidachalcone (3), a new sesquiterpene (4), and a previously reported pea flavonoid phytoalexin, pisatin (5). The structures of 1-4 were established by spectroscopic methods, including HREIMS, and 1H, 13C, DEPT, HMQC, and HMBC NMR experiments. The most potent estrogenic activity of these isolated natural products in an estrogen receptor (ERalpha) competitive-binding assay was for 3, which exhibited an IC50 value of 80 muM, compared with 18 nM for the natural steroid 17beta-estradiol. Results were interpreted via virtual docking of isolated compounds to an ERalpha crystal structure.

DrugBank Data that Cites this Article

Binding Properties
DrugTargetPropertyMeasurementpHTemperature (°C)
EstradiolEstrogen receptor alphaIC 50 (nM)18N/AN/ADetails