Carbonic anhydrase VII is S-glutathionylated without loss of catalytic activity and affinity for sulfonamide inhibitors.

Article Details

Citation

Truppo E, Supuran CT, Sandomenico A, Vullo D, Innocenti A, Di Fiore A, Alterio V, De Simone G, Monti SM

Carbonic anhydrase VII is S-glutathionylated without loss of catalytic activity and affinity for sulfonamide inhibitors.

Bioorg Med Chem Lett. 2012 Feb 15;22(4):1560-4. doi: 10.1016/j.bmcl.2011.12.134. Epub 2012 Jan 5.

PubMed ID
22277279 [ View in PubMed
]
Abstract

Human carbonic anhydrase (CA, EC 4.2.1.1) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Here we report an unexpected S-glutathionylation of hCA VII which has also been observed earlier in vivo for hCA III, another cytosolic isoform. Cys183 and Cys217 were found to be the residues involved in reaction with glutathione for hCA VII. The two reactive cysteines were then mutated and the corresponding variant (C183S/C217S) expressed. The native enzyme, the variant and the S-glutathionylated adduct (sgCA VII) as well as hCA III were fully characterized for their CO(2) hydration, esterase/phosphatase activities, and inhibition with sulfonamides. Our findings suggest that hCA VII could use the in vivo S-glutathionylation to function as an oxygen radical scavenger for protecting cells from oxidative damage, as the activity and affinity for inhibitors of the modified enzyme are similar to those of the wild type.

DrugBank Data that Cites this Article

Binding Properties
DrugTargetPropertyMeasurementpHTemperature (°C)
AcetazolamideCarbonic anhydrase 3Ki (nM)240000N/AN/ADetails
AcetazolamideCarbonic anhydrase 3IC 50 (nM)840N/AN/ADetails
AcetazolamideCarbonic anhydrase 3IC 50 (nM)6500N/AN/ADetails
AcetazolamideCarbonic anhydrase 7IC 50 (nM)265N/AN/ADetails
AcetazolamideCarbonic anhydrase 7IC 50 (nM)3440N/AN/ADetails
AcetazolamideCarbonic anhydrase 7IC 50 (nM)2520N/AN/ADetails
AcetazolamideCarbonic anhydrase 7Ki (nM)2.8N/AN/ADetails
AcetazolamideCarbonic anhydrase 7Ki (nM)2.7N/AN/ADetails
AcetazolamideCarbonic anhydrase 7IC 50 (nM)357N/AN/ADetails