Novel prostaglandin D synthase inhibitors generated by fragment-based drug design.

Article Details

Citation

Hohwy M, Spadola L, Lundquist B, Hawtin P, Dahmen J, Groth-Clausen I, Nilsson E, Persdotter S, von Wachenfeldt K, Folmer RH, Edman K

Novel prostaglandin D synthase inhibitors generated by fragment-based drug design.

J Med Chem. 2008 Apr 10;51(7):2178-86. doi: 10.1021/jm701509k. Epub 2008 Mar 15.

PubMed ID
18341273 [ View in PubMed
]
Abstract

We describe the discovery of novel inhibitors of prostaglandin D2 synthase (PGDS) through fragment-based lead generation and structure-based drug design. A library of 2500 low-molecular-weight compounds was screened using 2D nuclear magnetic resonance (NMR), leading to the identification of 24 primary hits. Structure determination of protein-ligand complexes with the hits enabled a hit optimization process, whereby we harvested increasingly more potent inhibitors out of our corporate compound collection. Two iterative cycles were carried out, comprising NMR screening, molecular modeling, X-ray crystallography, and in vitro biochemical testing. Six novel high-resolution PGDS complex structures were determined, and 300 hit analogues were tested. This rational drug design procedure culminated in the discovery of 24 compounds with an IC 50 below 1 microM in the in vitro assay. The best inhibitor (IC 50 = 21 nM) is one of the most potent inhibitors of PGDS to date. As such, it may enable new functional in vivo studies of PGDS and the prostaglandin metabolism pathway.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Hematopoietic prostaglandin D synthaseO60760Details
Binding Properties
DrugTargetPropertyMeasurementpHTemperature (°C)
1-PHENYL-1H-PYRAZOLE-4-CARBOXYLIC ACIDHematopoietic prostaglandin D synthaseIC 50 (nM)8900007.222Details
3-phenyl-5-(1H-pyrazol-3-yl)isoxazoleHematopoietic prostaglandin D synthaseIC 50 (nM)9207.222Details
4-{[4-(4-fluoro-3-methylphenyl)-1,3-thiazol-2-yl]amino}-2-hydroxybenzoic acidHematopoietic prostaglandin D synthaseIC 50 (nM)1387.222Details
PHENYL-5-(1H-PYRAZOL-3-YL)-1,3-THIAZOLEHematopoietic prostaglandin D synthaseIC 50 (nM)217.222Details
TranilastHematopoietic prostaglandin D synthaseIC 50 (nM)116007.222Details