Development of a real-time in vivo transcription assay: application reveals pregnane X receptor-mediated induction of CYP3A4 by cancer chemotherapeutic agents.
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Schuetz E, Lan L, Yasuda K, Kim R, Kocarek TA, Schuetz J, Strom S
Development of a real-time in vivo transcription assay: application reveals pregnane X receptor-mediated induction of CYP3A4 by cancer chemotherapeutic agents.
Mol Pharmacol. 2002 Sep;62(3):439-45.
- PubMed ID
- 12181418 [ View in PubMed]
- Abstract
We report the development of a rapid real-time assay that measures the transcription of luciferase reporter genes in transduced mouse hepatic cells in vivo. Luciferase activity is noninvasively measured by whole-body optical imaging within hours of the hydrodynamic injection of as little as 1 microg of naked DNA. Transcription of genes introduced as linearized DNA can be serially assayed for weeks in each animal. Transcription was quantified by extracorporal monitoring of bioluminescence as well as or better than by traditional in vitro bioluminescence assay. Our assay allows the measurement of transcription as it occurs, under the most informative biological conditions (i.e., in a living, intact organ). Furthermore, it substantially reduces the cost, time, and number of animals required for analysis of gene expression. The utility of the method is demonstrated in the discovery that topotecan and etoposide are ligands of pregnane X receptor that induce CYP3A4 transcription.
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Etoposide Cytochrome P450 3A4 Protein Humans UnknownSubstrateInhibitorInducerDetails - Drug Interactions
Drugs Interaction Integrate drug-drug
interactions in your softwareAcenocoumarolEtoposide The therapeutic efficacy of Acenocoumarol can be increased when used in combination with Etoposide. DicoumarolEtoposide The therapeutic efficacy of Dicoumarol can be increased when used in combination with Etoposide. FluindioneEtoposide The therapeutic efficacy of Fluindione can be increased when used in combination with Etoposide. PhenindioneEtoposide The therapeutic efficacy of Phenindione can be increased when used in combination with Etoposide. PhenprocoumonEtoposide The therapeutic efficacy of Phenprocoumon can be increased when used in combination with Etoposide.