Rat hepatic CYP1A1 and CYP1A2 induction by menadione.

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Sidorova YA, Grishanova AY, Lyakhovich VV

Rat hepatic CYP1A1 and CYP1A2 induction by menadione.

Toxicol Lett. 2005 Feb 15;155(2):253-8. doi: 10.1016/j.toxlet.2004.10.001.

PubMed ID

15603920 [ View in PubMed

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Abstract

The effects of menadione on activities and expression of cytochrome P450 (CYP) 1A subfamily (CYP1A) isozymes in rat hepatic tissue were examined. When rats were treated orally with 15 mg/kg menadione for 4 days, the elevation of hepatic CYP1A1/1A2 specific activities in microsomal preparations was detected with approximately 5.4- and 2.5-fold increase over control values for ethoxyresorufin-O-deethylase (EROD, CYP1A1) and methoxyresorufin-O-demethylase (MROD, CYP1A2) activities, respectively. CYP1A1 and CYP1A2 mRNA levels in the liver of menadione-treated rats were approximately 11.8- and 1.8-fold higher than in controls, respectively, whereas the expression of the CYP1A regulatory proteins aryl hydrocarbon-receptor (AhR) and AhR nuclear translocator (Arnt) was not changed at the mRNA level. The result of this study demonstrates that menadione induces CYP1A1/1A2 expression in vivo through either transcriptional activation and/or mRNA stabilization.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Menadione	Cytochrome P450 1A1	Protein	Humans	Unknown	Inhibitor Inducer	Details
Menadione	Cytochrome P450 1A2	Protein	Humans	Unknown	Inhibitor Inducer	Details