Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes.
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Schiltz E, Reinbolt J
Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes.
Eur J Biochem. 1975 Aug 15;56(2):467-81.
- PubMed ID
- 1100394 [ View in PubMed]
- Abstract
After digestion of protein S4 with trypsin, all 32 tryptic peptides were isolated. Their amino acid compositions were analyzed and the sequence of the amino acids within the tryptic peptides was determined by means of a solid-phase peptide sequenator and by exopeptidases. Alignment of the tryptic peptides was established by analyzing and partially sequencing peptides isolated after digestion of the S4 protein with chymotrypsin, thermolysin and a glutamic-acid-specific protease. Further information about the alignment of peptides came from treatment of S4 with CNBr and with a lysine-modifying reagent.