Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.

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Arnold RJ, Reilly JP

Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.

Anal Biochem. 1999 Apr 10;269(1):105-12.

PubMed ID
10094780 [ View in PubMed
]
Abstract

Ribosomes from the K-12 strain of Escherichia coli were analyzed with good sensitivity and high mass accuracy using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Fifty-five of the 56 subunit proteins were observable. Mass spectral peak locations were consistent with previously reported post-translational modifications involving N-terminal methionine loss, methylation, thiomethylation, and acetylation for all but one case. The speed and accuracy of mass spectrometry make it a good candidate for phylogenetic studies of ribosomes and the observation of posttranslational modifications in other organisms.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
30S ribosomal protein S4P0A7V8Details
30S ribosomal protein S9P0A7X3Details
30S ribosomal protein S10P0A7R5Details
30S ribosomal protein S12P0A7S3Details
50S ribosomal protein L4P60723Details
30S ribosomal protein S14P0AG59Details
30S ribosomal protein S13P0A7S9Details
30S ribosomal protein S19P0A7U3Details
50S ribosomal protein L16P0ADY7Details
30S ribosomal protein S3P0A7V3Details
30S ribosomal protein S8P0A7W7Details
30S ribosomal protein S7P02359Details