Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
Article Details
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Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J
Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
Cell. 2003 Jun 13;113(6):789-801.
- PubMed ID
- 12809609 [ View in PubMed]
- Abstract
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID 30S ribosomal protein S4 P0A7V8 Details 30S ribosomal protein S9 P0A7X3 Details 30S ribosomal protein S10 P0A7R5 Details 30S ribosomal protein S12 P0A7S3 Details 50S ribosomal protein L4 P60723 Details 30S ribosomal protein S14 P0AG59 Details 30S ribosomal protein S13 P0A7S9 Details 30S ribosomal protein S19 P0A7U3 Details 50S ribosomal protein L16 P0ADY7 Details 30S ribosomal protein S3 P0A7V3 Details 30S ribosomal protein S8 P0A7W7 Details 30S ribosomal protein S7 P02359 Details