Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.

Article Details

Citation

Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J

Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.

Cell. 2003 Jun 13;113(6):789-801.

PubMed ID
12809609 [ View in PubMed
]
Abstract

Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
30S ribosomal protein S4P0A7V8Details
30S ribosomal protein S9P0A7X3Details
30S ribosomal protein S10P0A7R5Details
30S ribosomal protein S12P0A7S3Details
50S ribosomal protein L4P60723Details
30S ribosomal protein S14P0AG59Details
30S ribosomal protein S13P0A7S9Details
30S ribosomal protein S19P0A7U3Details
50S ribosomal protein L16P0ADY7Details
30S ribosomal protein S3P0A7V3Details
30S ribosomal protein S8P0A7W7Details
30S ribosomal protein S7P02359Details