Partial amino acid sequence analyses of human placental insulin receptor.
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Fujita-Yamaguchi Y, Hawke DH, Shively JE, Choi S
Partial amino acid sequence analyses of human placental insulin receptor.
Protein Seq Data Anal. 1987;1(1):3-6.
- PubMed ID
- 3447155 [ View in PubMed]
- Abstract
Tryptic peptides were isolated from human insulin receptor (1.8 nmol) by sequential reverse-phase HPLC on SynChropak RP-C8 and Vydac C-18. Nearly 20 peptides were analyzed for amino acid composition, which revealed that the yield of tryptic peptides averaged 300 pmol. By gas-phase microsequencing five peptides were sequenced. N-acetylglucosamine was found in two peptides. These data are compared with the amino acid sequence of the insulin receptor deduced from cloned cDNA encoding for human insulin receptor.