Phosphorylation and regulation of Raf by Akt (protein kinase B).

Article Details


Zimmermann S, Moelling K

Phosphorylation and regulation of Raf by Akt (protein kinase B).

Science. 1999 Nov 26;286(5445):1741-4.

PubMed ID
10576742 [ View in PubMed

Activation of the protein kinase Raf can lead to opposing cellular responses such as proliferation, growth arrest, apoptosis, or differentiation. Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. This phosphorylation of Raf by Akt inhibited activation of the Raf-MEK-ERK signaling pathway and shifted the cellular response in a human breast cancer cell line from cell cycle arrest to proliferation. These observations provide a molecular basis for cross talk between two signaling pathways at the level of Raf and Akt.

DrugBank Data that Cites this Article

NameUniProt ID
RAF proto-oncogene serine/threonine-protein kinaseP04049Details
RAC-alpha serine/threonine-protein kinaseP31749Details