Molecular cloning of a non-isopeptide-selective human endothelin receptor.

Article Details

Citation

Ogawa Y, Nakao K, Arai H, Nakagawa O, Hosoda K, Suga S, Nakanishi S, Imura H

Molecular cloning of a non-isopeptide-selective human endothelin receptor.

Biochem Biophys Res Commun. 1991 Jul 15;178(1):248-55.

PubMed ID
1648908 [ View in PubMed
]
Abstract

We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3'-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Endothelin B receptorP24530Details