Quantitative detection of single amino acid polymorphisms by targeted proteomics.

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Su ZD, Sun L, Yu DX, Li RX, Li HX, Yu ZJ, Sheng QH, Lin X, Zeng R, Wu JR

Quantitative detection of single amino acid polymorphisms by targeted proteomics.

J Mol Cell Biol. 2011 Oct;3(5):309-15. doi: 10.1093/jmcb/mjr024.

PubMed ID
22028381 [ View in PubMed
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Abstract

Single-nucleotide polymorphisms (SNPs) are recognized as one kind of major genetic variants in population scale. However, polymorphisms at the proteome level in population scale remain elusive. In the present study, we named amino acid variances derived from SNPs within coding regions as single amino acid polymorphisms (SAPs) at the proteome level, and developed a pipeline of non-targeted and targeted proteomics to identify and quantify SAP peptides in human plasma. The absolute concentrations of three selected SAP-peptide pairs among 290 Asian individuals were measured by selected reaction monitoring (SRM) approach, and their associations with both obesity and diabetes were further analyzed. This work revealed that heterozygotes and homozygotes with various SAPs in a population could have different associations with particular traits. In addition, the SRM approach allows us for the first time to separately measure the absolute concentration of each SAP peptide in the heterozygotes, which also shows different associations with particular traits.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
ProthrombinP00734Details
Complement C5P01031Details
Apolipoprotein EP02649Details
Complement C1r subcomponentP00736Details
Apolipoprotein A-IP02647Details
Complement C3P01024Details
Complement factor HP08603Details
Pigment epithelium-derived factorP36955Details
Apolipoprotein B-100P04114Details