A genome annotation-driven approach to cloning the human ORFeome.

Article Details


Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I

A genome annotation-driven approach to cloning the human ORFeome.

Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30.

PubMed ID
15461802 [ View in PubMed

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

DrugBank Data that Cites this Article

NameUniProt ID
Cationic amino acid transporter 4O43246Details
Peroxisome proliferator-activated receptor alphaQ07869Details
NADH-cytochrome b5 reductase 3P00387Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6P56556Details
Choline/ethanolamine kinaseQ9Y259Details
SEC14-like protein 2O76054Details
Interleukin-2 receptor subunit betaP14784Details
Phosphatidylserine decarboxylase proenzymeQ9UG56Details
Heme oxygenase 1P09601Details
Translocator proteinP30536Details
Heparin cofactor 2P05546Details
Glutathione S-transferase theta-1P30711Details
Gamma-glutamyltranspeptidase 1P19440Details
Adenosine receptor A2aP29274Details
85/88 kDa calcium-independent phospholipase A2O60733Details
Tubulin alpha-8 chainQ9NY65Details
Macrophage migration inhibitory factorP14174Details
Catechol O-methyltransferaseP21964Details
Cytokine receptor common subunit betaP32927Details
Mitochondrial glutamate carrier 2Q9H1K4Details
Mitogen-activated protein kinase 12P53778Details
Arylsulfatase AP15289Details
Metalloproteinase inhibitor 3P35625Details
Serine/threonine-protein kinase Chk2O96017Details
Mitogen-activated protein kinase 11Q15759Details
Melanin-concentrating hormone receptor 1Q99705Details
Platelet-derived growth factor subunit BP01127Details
Casein kinase I isoform epsilonP49674Details
Histone deacetylase 10Q969S8Details
Somatostatin receptor type 3P32745Details
60S ribosomal protein L3P39023Details
NHP2-like protein 1P55769Details
MKL/myocardin-like protein 1Q969V6Details
tRNA-splicing ligase RtcB homologQ9Y3I0Details
Meiotic recombination protein DMC1/LIM15 homologQ14565Details
Thiosulfate sulfurtransferaseQ16762Details
Parvalbumin alphaP20472Details
Aconitate hydratase, mitochondrialQ99798Details
Sodium/glucose cotransporter 1P13866Details
Ras-related C3 botulinum toxin substrate 2P15153Details
Neutrophil cytosol factor 4Q15080Details
Cyclic AMP-dependent transcription factor ATF-4P18848Details
Voltage-dependent calcium channel gamma-2 subunitQ9Y698Details
Inward rectifier potassium channel 4P48050Details
Solute carrier family 2, facilitated glucose transporter member 11Q9BYW1Details
Ran-specific GTPase-activating proteinP43487Details
14-3-3 protein etaQ04917Details
LIM domain kinase 2P53671Details
Sulfotransferase 4A1Q9BR01Details
P2X purinoceptor 6O15547Details
V-type proton ATPase subunit E 1P36543Details
Glutathione S-transferase theta-2BP0CG30Details