A genome annotation-driven approach to cloning the human ORFeome.

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Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I

A genome annotation-driven approach to cloning the human ORFeome.

Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30.

PubMed ID
15461802 [ View in PubMed
]
Abstract

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Cationic amino acid transporter 4O43246Details
Peroxisome proliferator-activated receptor alphaQ07869Details
NADH-cytochrome b5 reductase 3P00387Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6P56556Details
Choline/ethanolamine kinaseQ9Y259Details
SEC14-like protein 2O76054Details
Transcobalamin-2P20062Details
Interleukin-2 receptor subunit betaP14784Details
Phosphatidylserine decarboxylase proenzymeQ9UG56Details
Heme oxygenase 1P09601Details
Translocator proteinP30536Details
Heparin cofactor 2P05546Details
Glutathione S-transferase theta-1P30711Details
Gamma-glutamyltranspeptidase 1P19440Details
Adenosine receptor A2aP29274Details
85/88 kDa calcium-independent phospholipase A2O60733Details
Tubulin alpha-8 chainQ9NY65Details
Macrophage migration inhibitory factorP14174Details
Catechol O-methyltransferaseP21964Details
Cytokine receptor common subunit betaP32927Details
Mitochondrial glutamate carrier 2Q9H1K4Details
Mitogen-activated protein kinase 12P53778Details
Arylsulfatase AP15289Details
Galectin-2P05162Details
Metalloproteinase inhibitor 3P35625Details
Serine/threonine-protein kinase Chk2O96017Details
Mitogen-activated protein kinase 11Q15759Details
Melanin-concentrating hormone receptor 1Q99705Details
Platelet-derived growth factor subunit BP01127Details
Casein kinase I isoform epsilonP49674Details
Histone deacetylase 10Q969S8Details
Somatostatin receptor type 3P32745Details
60S ribosomal protein L3P39023Details
NHP2-like protein 1P55769Details
MKL/myocardin-like protein 1Q969V6Details
tRNA-splicing ligase RtcB homologQ9Y3I0Details
Meiotic recombination protein DMC1/LIM15 homologQ14565Details
Thiosulfate sulfurtransferaseQ16762Details
MyoglobinP02144Details
Parvalbumin alphaP20472Details
Galectin-1P09382Details
Aconitate hydratase, mitochondrialQ99798Details
Alpha-N-acetylgalactosaminidaseP17050Details
Sodium/glucose cotransporter 1P13866Details
Ras-related C3 botulinum toxin substrate 2P15153Details
Neutrophil cytosol factor 4Q15080Details
Cyclic AMP-dependent transcription factor ATF-4P18848Details
Voltage-dependent calcium channel gamma-2 subunitQ9Y698Details
Inward rectifier potassium channel 4P48050Details
Solute carrier family 2, facilitated glucose transporter member 11Q9BYW1Details
Ran-specific GTPase-activating proteinP43487Details
14-3-3 protein etaQ04917Details
Myosin-9P35579Details
Beta-ureidopropionaseQ9UBR1Details
LIM domain kinase 2P53671Details
Sulfotransferase 4A1Q9BR01Details
P2X purinoceptor 6O15547Details
V-type proton ATPase subunit E 1P36543Details
Glutathione S-transferase theta-2BP0CG30Details