Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

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Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O

Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

DNA Res. 1998 Oct 30;5(5):277-86.

PubMed ID
9872452 [ View in PubMed
]
Abstract

In our series of projects for accumulating sequence information on the coding sequences of unidentified human genes, we have newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0711 to KIAA0810. These cDNA clones were selected according to their coding potentials of large proteins (50 kDa and more) in vitro. The average sizes of the inserts and corresponding open reading frames were 4.3 kb and 2.6 kb (869 amino acid residues), respectively. Sequence analyses against the public databases indicated that the predicted coding sequences of 78 genes were similar to those of known genes, 64% of which (50 genes) were categorized as proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. As additional information concerning genes characterized in this study, the chromosomal locations of the clones were determined by using human-rodent hybrid panels and the expression profiles among 10 human tissues were examined by reverse transcription-coupled polymerase chain reaction which was substantially improved by enzyme-linked immunosorbent assay.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Histone deacetylase 9Q9UKV0Details
Synaptic vesicle glycoprotein 2AQ7L0J3Details
Telomerase-binding protein EST1AQ86US8Details
Electroneutral sodium bicarbonate exchanger 1Q2Y0W8Details
Sodium/potassium-transporting ATPase subunit alpha-2P50993Details
Serine/threonine-protein kinase ULK1O75385Details
Calcium/calmodulin-dependent protein kinase kinase 2Q96RR4Details
Serine/threonine-protein kinase SIK2Q9H0K1Details
Diacylglycerol kinase betaQ9Y6T7Details