Stoichiometry of the human glycine receptor revealed by direct subunit counting.
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Durisic N, Godin AG, Wever CM, Heyes CD, Lakadamyali M, Dent JA
Stoichiometry of the human glycine receptor revealed by direct subunit counting.
J Neurosci. 2012 Sep 12;32(37):12915-20. doi: 10.1523/JNEUROSCI.2050-12.2012.
- PubMed ID
- 22973015 [ View in PubMed]
- Abstract
The subunit stoichiometry of heteromeric glycine-gated channels determines fundamental properties of these key inhibitory neurotransmitter receptors; however, the ratio of alpha1- to beta-subunits per receptor remains controversial. We used single-molecule imaging and stepwise photobleaching in Xenopus oocytes to directly determine the subunit stoichiometry of a glycine receptor to be 3alpha1:2beta. This approach allowed us to determine the receptor stoichiometry in mixed populations consisting of both heteromeric and homomeric channels, additionally revealing the quantitative proportions for the two populations.