Cloning and characterization of a human angiotensin II type 1 receptor.

Article Details

Citation

Bergsma DJ, Ellis C, Kumar C, Nuthulaganti P, Kersten H, Elshourbagy N, Griffin E, Stadel JM, Aiyar N

Cloning and characterization of a human angiotensin II type 1 receptor.

Biochem Biophys Res Commun. 1992 Mar 31;183(3):989-95.

PubMed ID
1567413 [ View in PubMed
]
Abstract

A human liver cDNA library was screened using a rat type 1 angiotensin II receptor cDNA coding sequence as a probe. cDNA clones were isolated which encoded a protein of 359 amino acids that shared 94.4% and 95.3% identify to rat and bovine type 1 angiotensin II receptors, respectively. Ligand binding studies of the cloned receptor expressed in COS cells suggested that it is pharmacologically a type 1 angiotensin II receptor subtype. Electrophysiological studies of the receptor expressed in Xenopus laevis oocytes revealed that it could functionally couple to a second messenger system leading to the mobilization of intracellular stores of calcium. Southern and Northern blot analyses indicated that the cloned receptor is represented as a single copy in the human genome and is expressed in many tissues of different histogenic origin with the exception of brain, where mRNA transcripts were barely detectable.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Type-1 angiotensin II receptorP30556Details