Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.
Article Details
- CitationCopy to clipboard
Koike K, Urata Y, Koike M
Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5594-7.
- PubMed ID
- 2377599 [ View in PubMed]
- Abstract
A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.