Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.

Article Details


Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.

Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.

DNA Res. 1997 Apr 28;4(2):91-113.

PubMed ID
9205837 [ View in PubMed

The contiguous 874.423 base pair sequence corresponding to the 50.0-68.8 min region on the genetic map of the Escherichia coli K-12 (W3110) was constructed by the determination of DNA sequences in the 50.0-57.9 min region (360 kb) and two large (100 kb in all) and five short gaps in the 57.9-68.8 min region whose sequences had been registered in the DNA databases. We analyzed its sequence features and found that this region contained at least 894 potential open reading frames (ORFs), of which 346 (38.7%) were previously reported, 158 (17.7%) were homologous to other known genes, 232 (26.0%) were identical or similar to hypothetical genes registered in databases, and the remaining 158 (17.7%) showed no significant similarity to any other genes. A homology search of the ORFs also identified several new gene clusters. Those include two clusters of fimbrial genes, a gene cluster of three genes encoding homologues of the human long chain fatty acid degradation enzyme complex in the mitochondrial membrane, a cluster of at least nine genes involved in the utilization of ethanolamine, a cluster of the secondary set of 11 hyc genes participating in the formate hydrogenlyase reaction and a cluster of five genes coding for the homologues of degradation enzymes for aromatic hydrocarbons in Pseudomonas putida. We also noted a variety of novel genes, including two ORFs, which were homologous to the putative genes encoding xanthine dehydrogenase in the fly and a protein responsible for axonal guidance and outgrowth of the rat, mouse and nematode. An isoleucine tRNA gene, designated ileY, was also newly identified at 60.0 min.

DrugBank Data that Cites this Article

NameUniProt ID
Bifunctional protein FolCP08192Details
3-oxoacyl-[acyl-carrier-protein] synthase 1P0A953Details
Phosphoribosylglycinamide formyltransferaseP08179Details
4-aminobutyrate aminotransferase GabTP22256Details
Purine nucleoside phosphorylase 2P45563Details
Membrane-bound lytic murein transglycosylase BP41052Details
Long-chain fatty acid transport proteinP10384Details
Glutamate--cysteine ligaseP0A6W9Details
Bifunctional polymyxin resistance protein ArnAP77398Details
Histidine--tRNA ligaseP60906Details
Penicillin-insensitive murein endopeptidaseP0C0T5Details
Glucarate dehydrataseP0AES2Details
Glycine betaine-binding periplasmic proteinP0AFM2Details
GMP synthase [glutamine-hydrolyzing]P04079Details
o-succinylbenzoate synthaseP29208Details
Laccase domain protein YfiHP33644Details
3-mercaptopyruvate sulfurtransferaseP31142Details
Ribonucleoside-diphosphate reductase 1 subunit betaP69924Details
Penicillin-binding protein 1CP76577Details
Cell division protein ZipAP77173Details
DNA gyrase subunit AP0AES4Details