Cloning of human androgen receptor complementary DNA and localization to the X chromosome.
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Lubahn DB, Joseph DR, Sullivan PM, Willard HF, French FS, Wilson EM
Cloning of human androgen receptor complementary DNA and localization to the X chromosome.
Science. 1988 Apr 15;240(4850):327-30.
- PubMed ID
- 3353727 [ View in PubMed]
- Abstract
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors. The AR gene was localized on the human X chromosome between the centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, was expressed in monkey kidney (COS) cells and yielded a high-affinity androgen-binding protein with steroid-binding specificity corresponding to that of native AR. A predominant messenger RNA species of 9.6 kilobases was identified in human, rat, and mouse tissues known to contain AR and was undetectable in tissues lacking AR androgen-binding activity, including kidney and liver from androgen-insensitive mice. The deduced amino acid sequence of AR within the DNA-binding domain has highest sequence identity with the progesterone receptor.