Microsatellite mutation (CAG24-->18) in the androgen receptor gene in human prostate cancer.

Article Details

Citation

Schoenberg MP, Hakimi JM, Wang S, Bova GS, Epstein JI, Fischbeck KH, Isaacs WB, Walsh PC, Barrack ER

Microsatellite mutation (CAG24-->18) in the androgen receptor gene in human prostate cancer.

Biochem Biophys Res Commun. 1994 Jan 14;198(1):74-80.

PubMed ID
8292051 [ View in PubMed
]
Abstract

The androgen receptor (AR) gene contains a polymorphic CAG microsatellite that codes for a variable length of glutamine repeats in the AR protein. Microsatellite DNA sequences may be potential sites of genetic instability. Using the polymerase chain reaction (PCR), we screened 40 human prostate cancer specimens for expansions or deletions of this microsatellite. In one patient, nontumor DNA yielded a single PCR product, as expected for the AR, but the tumor DNA yielded two discrete products, one identical to normal, and a second smaller one. Direct sequencing revealed that the nontumor tissue contained 24 CAGs, whereas the tumor contained one fragment with 24 CAGs (wild-type) and a second fragment with 18 CAGs (mutant), representing a somatic contraction of the AR CAG repeat (CAG24-->CAG18) in the tumor. Interestingly, this patient manifested a paradoxical agonistic response to hormonal therapy with the antiandrogen flutamide.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Androgen receptorP10275Details