Molecular cloning and expression of human cGMP-binding cGMP-specific phosphodiesterase (PDE5).
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Stacey P, Rulten S, Dapling A, Phillips SC
Molecular cloning and expression of human cGMP-binding cGMP-specific phosphodiesterase (PDE5).
Biochem Biophys Res Commun. 1998 Jun 18;247(2):249-54.
- PubMed ID
- 9642111 [ View in PubMed]
- Abstract
A human PDE5 cDNA has been isolated which contains an open reading frame encoding an 875 amino acid, 100,012 Da polypeptide, the expression of which yields a protein of the predicted size and is capable of hydrolyzing cGMP. The deduced amino acid sequence is very similar (95%) to that of bovine PDE5, and comprises a conserved cGMP-binding domain and catalytic domain. Northern analysis reveals a major and minor transcript of approximately 9 kb and approximately 8 kb respectively, thus indicating the existence of at least two splice variants, the major form being readily detected in bladder, colon, lung, pancreas, placenta, prostate, small intestine, and stomach.