Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.
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Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O
Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.
DNA Res. 2000 Feb 28;7(1):65-73.
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- 10718198 [ View in PubMed]
- Abstract
We have carried out a human cDNA sequencing project to accumulate information regarding the coding sequences of unidentified human genes. As an extension of the preceding reports, we herein present the entire sequences of 150 cDNA clones of unknown human genes, named KIAA1294 to KIAA1443, from two sets of size-fractionated human adult and fetal brain cDNA libraries. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyzed here reached 4.8 kb and 2.7 kb (910 amino acid residues), respectively. From sequence similarities and protein motifs, 73 predicted gene products were functionally annotated and 97% of them were classified into the following four functional categories: cell signaling/communication, nucleic acid management, cell structure/motility and protein management. Additionally, the chromosomal loci of the genes were assigned by using human-rodent hybrid panels for those genes whose mapping data were not available in the public databases. The expression profiles of the genes were also studied in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Calcium-transporting ATPase type 2C member 1 P98194 Details Leucine--tRNA ligase, cytoplasmic Q9P2J5 Details Sodium channel protein type 3 subunit alpha Q9NY46 Details Sphingomyelin phosphodiesterase 4 Q9NXE4 Details E3 ubiquitin-protein ligase UBR4 Q5T4S7 Details Gephyrin Q9NQX3 Details Ribosome-binding protein 1 Q9P2E9 Details MKL/myocardin-like protein 1 Q969V6 Details Protocadherin-19 Q8TAB3 Details KAT8 regulatory NSL complex subunit 3 Q9P2N6 Details Serine/threonine-protein kinase TAO1 Q7L7X3 Details Eukaryotic translation initiation factor 2-alpha kinase 1 Q9BQI3 Details eIF-2-alpha kinase GCN2 Q9P2K8 Details Sodium-coupled neutral amino acid transporter 2 Q96QD8 Details Cell surface hyaluronidase Q9UHN6 Details