Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.

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Citation

Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O

Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.

DNA Res. 2000 Feb 28;7(1):65-73.

PubMed ID
10718198 [ View in PubMed
]
Abstract

We have carried out a human cDNA sequencing project to accumulate information regarding the coding sequences of unidentified human genes. As an extension of the preceding reports, we herein present the entire sequences of 150 cDNA clones of unknown human genes, named KIAA1294 to KIAA1443, from two sets of size-fractionated human adult and fetal brain cDNA libraries. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyzed here reached 4.8 kb and 2.7 kb (910 amino acid residues), respectively. From sequence similarities and protein motifs, 73 predicted gene products were functionally annotated and 97% of them were classified into the following four functional categories: cell signaling/communication, nucleic acid management, cell structure/motility and protein management. Additionally, the chromosomal loci of the genes were assigned by using human-rodent hybrid panels for those genes whose mapping data were not available in the public databases. The expression profiles of the genes were also studied in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Calcium-transporting ATPase type 2C member 1P98194Details
Leucine--tRNA ligase, cytoplasmicQ9P2J5Details
Sodium channel protein type 3 subunit alphaQ9NY46Details
Sphingomyelin phosphodiesterase 4Q9NXE4Details
E3 ubiquitin-protein ligase UBR4Q5T4S7Details
GephyrinQ9NQX3Details
Ribosome-binding protein 1Q9P2E9Details
MKL/myocardin-like protein 1Q969V6Details
Protocadherin-19Q8TAB3Details
KAT8 regulatory NSL complex subunit 3Q9P2N6Details
Serine/threonine-protein kinase TAO1Q7L7X3Details
Eukaryotic translation initiation factor 2-alpha kinase 1Q9BQI3Details
eIF-2-alpha kinase GCN2Q9P2K8Details
Sodium-coupled neutral amino acid transporter 2Q96QD8Details
Cell surface hyaluronidaseQ9UHN6Details