Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.

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Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J

Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.

Electrophoresis. 1992 Dec;13(12):960-9.

PubMed ID
1286667 [ View in PubMed
]
Abstract

Microsequencing of proteins recovered from two-dimensional (2-D) gels is being used systematically to identify proteins in the master human keratinocyte 2-D gel database. To date, about 250 protein spots recorded in human 2-D gel databases have been microsequenced and, of these, 145 are recorded in the keratinocyte database under the entry partial amino acid sequence. Coomassie Brilliant Blue-stained protein spots cut from several (up to 40) dry gels were concentrated by elution-concentration gel electrophoresis, electroblotted onto PVDF membranes and digested in situ with trypsin. Eluting peptides were separated by reversed-phase HPLC, collected individually and sequenced. Computer search using the FASTA and TFASTA programs from Genetics Computer Group indicated that 110 of the microsequenced polypeptides shared significant similarity with proteins contained in the PIR, Mipsx or GenEMBL databases. Only 35 polypeptides corresponded to hitherto unknown proteins. Peptide sequences of all 145 proteins are listed together with their coordinates (apparent molecular weight and pI) in the keratinocyte database.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Tryptophan--tRNA ligase, cytoplasmicP23381Details
Plasminogen activator inhibitor 2P05120Details
CalnexinP27824Details
Bifunctional purine biosynthesis protein ATICP31939Details
Peroxiredoxin-2P32119Details
Fatty acid-binding protein 5Q01469Details
CalreticulinP27797Details
Peptidyl-prolyl cis-trans isomerase BP23284Details
Proteasome subunit beta type-2P49721Details
Protein S100-A11P31949Details
Protein S100-A2P29034Details
Protein S100-A7P31151Details
Heat shock cognate 71 kDa proteinP11142Details
Proteasome subunit alpha type-5P28066Details
Proteasome subunit beta type-3P49720Details
Proteasome subunit beta type-4P28070Details
Ubiquitin carboxyl-terminal hydrolase isozyme L1P09936Details
SorcinP30626Details
Translationally-controlled tumor proteinP13693Details
Protein S100-A8P05109Details
Heterogeneous nuclear ribonucleoprotein HP31943Details
Heterogeneous nuclear ribonucleoprotein H3P31942Details
Heterogeneous nuclear ribonucleoprotein LP14866Details
Stress-induced-phosphoprotein 1P31948Details
Proteasome activator complex subunit 1Q06323Details
Protein disulfide-isomerase A3P30101Details
Protein S100-A9P06702Details
Tropomyosin alpha-3 chainP06753Details
Tropomyosin alpha-4 chainP67936Details
Keratin, type I cytoskeletal 10P13645Details
Keratin, type I cytoskeletal 16P08779Details