Elimination of plasmids by enoxacin and ofloxacin at near inhibitory concentrations.

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Weisser J, Wiedemann B

Elimination of plasmids by enoxacin and ofloxacin at near inhibitory concentrations.

J Antimicrob Chemother. 1986 Nov;18(5):575-83.

PubMed ID

3542924 [ View in PubMed

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Abstract

The abilities of the 4-quinolones enoxacin and ofloxacin, inhibitors of DNA gyrase subunit A, to eliminate plasmids from Escherichia coli have been studied in a narrow concentration range just below the MIC. These compounds cured most efficiently at the highest concentration which still allows cell growth and produced 20% to 100% plasmid-free cells, depending upon the plasmid tested. Higher concentrations were required to eliminate plasmids from a gyrANalr strain, consistent with their higher MICs, but maximal curing frequencies were similar to those obtained with the Nals strain. Kinetics of plasmid elimination indicated that plasmid loss occurred by inhibition of plasmid replication, which seems to be somewhat more sensitive to the action of 4-quinolones than chromosome replication. Low or high curing frequencies with a given curing agent seem to be a property of the plasmid tested.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Enoxacin	DNA gyrase subunit A	Protein	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	Yes	Inhibitor	Details