Gene structure, polymorphism and mapping of the human endothelial nitric oxide synthase gene.

Article Details

Citation

Nadaud S, Bonnardeaux A, Lathrop M, Soubrier F

Gene structure, polymorphism and mapping of the human endothelial nitric oxide synthase gene.

Biochem Biophys Res Commun. 1994 Feb 15;198(3):1027-33.

PubMed ID
7509596 [ View in PubMed
]
Abstract

Endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) is synthesized from L-Arginine by the endothelial, constitutive, NO synthase. No facilitate genetic studies, we have cloned the human endothelial NO synthase gene and determined its structure. The gene is composed of 26 exons, ranging from 68 to 579 bp and spans 22 kb. We determined the transcription start point using human lung mRNA. No TATA-box was found at the expected distance from the transcription start point and several consensus sequences for transcription factors, including a shear-stress responsive element were identified in the 5'-flanking region. A highly polymorphic (CA) repeat within intron 13 was studied, allowing the precise genetic mapping of the gene to chromosome 7, within a 4 cM interval delimited by genethon markers AFM199Zd4 and AFM074Xg5.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Nitric oxide synthase, endothelialP29474Details