Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide.

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Di Fiore A, Monti SM, Hilvo M, Parkkila S, Romano V, Scaloni A, Pedone C, Scozzafava A, Supuran CT, De Simone G

Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide.

Proteins. 2009 Jan;74(1):164-75. doi: 10.1002/prot.22144.

PubMed ID
18618712 [ View in PubMed
]
Abstract

The cytosolic isoform XIII is a recently discovered member of the human carbonic anhydrase (hCA, EC 4.2.1.1) family. It is selectively expressed among other tissues in the reproductive organs, where it may control pH and ion balance regulation, ensuring thus proper fertilization conditions. The authors report here the X-ray crystallographic structure of this isozyme in the unbound state and in complex with a classical sulfonamide inhibitor, namely acetazolamide. A detailed comparison of the obtained structural data with those already reported for other CA isozymes provides novel insights into the catalytic properties of the members of this protein family. On the basis of the inhibitory properties of acetazolamide against various cytosolic/transmembrane isoforms and the structural differences detected within the active site of the various CA isoforms, further prospects for the design of isozyme-specific CA inhibitors are here proposed.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Carbonic anhydrase 1P00915Details
Carbonic anhydrase 2P00918Details
Carbonic anhydrase 4P22748Details
Carbonic anhydrase 12O43570Details
Carbonic anhydrase 3P07451Details
Carbonic anhydrase 5A, mitochondrialP35218Details
Carbonic anhydrase 13Q8N1Q1Details
Carbonic anhydrase 14Q9ULX7Details