All-trans retinoic acid is capable of inducing folate receptor beta expression in KG-1 cells.

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Citation

Xu Y, Wang T, Tang R, Tang S

All-trans retinoic acid is capable of inducing folate receptor beta expression in KG-1 cells.

Tumour Biol. 2010 Dec;31(6):589-95. doi: 10.1007/s13277-010-0074-0. Epub 2010 Jul 15.

PubMed ID
20632143 [ View in PubMed
]
Abstract

The high expression of folate receptor (FR) on cancer cells might be a potential target for cancer therapy. In this study, the FR-beta expression and the modulation effect of all-trans retinoic acid (ATRA) in a number of cancer cell lines were analyzed. The gateway of ATRA activity on FR-beta expression was further studied by a panel of retinoid activators and inhibitors. The results revealed that ATRA was capable of upregulating the expression of FR-beta protein in KG-1 cells in a dosage-dependent manner, not in KG-1a, NB4, HL60, 293, L1210, JAR, and Hela cells. FR-beta mRNA expression in KG-1 cells was higher when ATRA was present in culture medium at 10(-)(6) mol/L for 5 days, and it went down to baseline when ATRA was removed from the medium, vice versa. The upregulation of FR-beta expression in KG-1 cells by ATRA was not associated with cell proliferation and differentiation. In addition, activators of retinoid acid receptor (RAR)alpha and RARgamma, CD336, and CD2781 also induced FR-beta expression. The induction of FR-beta expression by CD336 could be inhibited by RARgamma antagonist CD2665; RARbeta agonist CD-417 and CD-2314 as well as retinoid X receptor (RXR) agonist LG100364 could not induce FR-beta expression. These results indicate that ATRA within a certain range of concentration could reversibly induce the expression of FR-beta in a dosage- and cell type-dependent manner, and its action in KG-1 cells might be associated with the signal transduction of retinoid receptor RARalpha and RARgamma, rather than RARbeta and RXRs.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Folate receptor betaP14207Details