Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase.

Article Details

Citation

Perry S, Harries H, Scholfield C, Lock T, King L, Gibson G, Goldfarb P

Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase.

FEBS Lett. 1995 Mar 6;360(3):277-80.

PubMed ID
7883047 [ View in PubMed
]
Abstract

Kidney cysteine conjugate beta-lyase (glutamine transaminase K, kyneurenine aminotransferase, EC 2.6.1.64) metabolises the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites which can produce nephrotoxicity and neurotoxicity in experimental animals and man. Using a combination of hybridisation screening and PCR techniques we have isolated a full-length cDNA for human kidney cysteine conjugate beta-lyase. Comparison of the deduced amino acid sequence with that of the rat enzyme indicated an 82% overall similarity, with 90% similarity around the pyridoxal phosphate binding site, many of the changes being conservative in nature. Expression of the cDNA in Cos-1 cells resulted in the production of a cytosolic enzyme which showed both cysteine conjugate beta-lyase and glutamine transminase K activity. Preliminary mapping of the gene for human cysteine conjugate beta-lyase by PCR analysis of genomic DNA from human-rodent hybrid cells indicated that it is located on human chromosome 9.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Kynurenine--oxoglutarate transaminase 1Q16773Details